Figure 3 | NPG Asia Materials

Figure 3

From: Bio-inspired micropatterned hydrogel to direct and deconstruct hierarchical processing of geometry-force signals by human mesenchymal stem cells during smooth muscle cell differentiation

Figure 3

Cellular anisotropy promotes the expression and incorporation of α-SMA protein to stress fibers. (a) Representative immunofluorescence images of the micropatterned cells co-stained for α-SMA (green), F-actin (red) and cell nucleus (blue). Scale bar=25 μm. Box-whisker plots showing the mean values of (b) α-SMA expression level and (c) colocalization of the α-SMA and F-actin fluorescence signals. Data represents mean±s.d. derived from 15 individually patterned cells from each experimental group. * denotes significant difference between the compared experimental groups by analysis of variance followed by least significant difference post hoc test. (P<0.05).

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