Figure 6 | NPG Asia Materials

Figure 6

From: Bio-inspired micropatterned hydrogel to direct and deconstruct hierarchical processing of geometry-force signals by human mesenchymal stem cells during smooth muscle cell differentiation

Figure 6

Phosphorylation of MRLC is cell shape-dependent and specific to SF subtypes. hMSCs patterned as (a) circle, (b) 1:1, (c) 5:1, (d) 10:1 and (e) 15:1 were fixed and co-stained for F-actin (gray), P-MRLC (red) and 2P-MRLC (green). Scale bar=10 μm. Right panel: Fluorescence-intensity profile graphs of F-actin, P-MRLC and 2P-MRLC across the x–y lines. Yellow arrows denote triple colocalization of all of the cytoskeletal proteins.

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