Table 1 Optimized parameters used in force volume measurements

From: Space-resolved quantitative mechanical measurements of soft and supersoft materials by atomic force microscopy

K (N m−1)

Tip radius (spherical)

Tip semiangle (sharp)

Lateral size (μm)

Resolution

Scan rate (Hz)

0.07

2550

17

20 × 20

64 × 64

0.07

vappr (μm s−1)

vret (μm s−1)

Ramp rate (Hz)

FC length (μm)

FC points

Force trigger (nN)

27–45

500–830

4.34

6–10

4096

20–5

Total time of measurement (min)

15

   
  1. Cells with double values represent ‘hard’ hydrogel-1 and ‘soft’ hydrogel-3 (softer). All parameters are explained in the Materials and methods section.
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