Figure 4

(a) UV-vis absorption spectrum of GdW10 NCs solution before and after the reduction. Inset: the corresponding digital images of before and after the reduction. (b) The temperature evaluation of pure NaCl solution and reduced GdW10@BSA/NCs (1 mg ml−1) under the irradiation of 808 nm laser with a power density of 1 W cm−2 as a functional of irradiation time (0–10 min); (c) cell viability assays on Hela cells incubated with reduced GdW10@BSA/NS solution (0, 62.5, 125, 250, 500 and 1000 μg ml−1) (c) before and (e) after 808 nm laser (1 W cm−2, 10 min); live-dead cell staining studies using Calcein-AM and PI on Hela cells incubated with reduced GdW10@BSA/NS solution (1000 μg ml−1) (d) before and (f) after 10-min irradiation with 808 nm laser (1 W cm−2, 10 min). Error bars were based on s.d. of six parallel samples. P-values were obtained by the Student’s test: **P<0.01, ***P<0.001.