Figure 9
(a) Image of intratumoral injection to a mouse bearing an A-253 tumor cell xenograft; (b) inhibitory effects on tumor growth of saline, single injection of free Cur, repeated injections of free Cur at days 4, 9 and 14, Cur-M alone and Cur-M-loaded CCH; (c) changes in tumor volume after intratumoral injection of saline, single injection of free Cur alone, repeated injections of free Cur alone, Cur-M alone and Cur-M-loaded CCH. Images were taken of tumor masses excised from mice bearing A-253 tumor cell xenografts at the time of euthanasia (1, 10 and 20 days (D) post-treatment). Each solution was injected into xenograft-bearing mice after the initial tumor had reached a volume of 150–200 mm3. Statistical analyses were performed using one-way analysis of variance with Bonferroni’s multiple comparison test (++P<0.001 versus the saline-injected group at day 20; +P<0.01 versus Cur-M-loaded CCH-injected group at day 10; #P<0.01 versus Cur-M-loaded CCH-injected group at 20 days.
