Figure 5
From: Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation
Characterization of the HiDFF-purified MPs using flow cytometry. (a) Representative field-emission SEM (FESEM) image of a HiDFF-purified MPs from whole blood. (b) Comparison of MPs isolation between the HiDFF- and centrifugation-based methods on EMPs (CD144+, CD41a−), LMPs (CD45+) and NMPs (CD66b+) in healthy subjects (n=4). The data are presented as the mean±s.d. (c) There is a strong correlation in the MPs quantification (slope: 1.25, R2: 0.9) between the centrifugation and HiDFF using EMPs and immune cell-derived MPs (CD45+, CD66b+, CD3+ and CD19+). (d) The association of HiDFF-purified LMPs and NMPs with body mass index (BMI), carotid intima-media thickness (CIMT) and triglyceride levels in T2DM patients (n=9).
