Figure 6

Using microscopic temperature-responsive gel particles for rupturing cancer cells. (a) Images of the cells and the RGD-modified microscopic temperature-responsive gel particles after incubating them at 37 °C for 24 h and (b) after subsequently expanding the gel particles at 22 °C for 35 min. Images of the cells and the gel particles located (c) in the center and (d) by the sides of the 24-well plates after expanding the gel particles and staining the remains of the cells with trypan blue. (e) Images of the cells and the gel particles that were not responsive to temperature after incubating them at 22 °C for 35 min. (f) Images of the cells and the gel particles that were not responsive to temperature after staining with trypan blue.