Figure 6

Relative cell viability of bone tumor cells (Sao-2) treating with different conditions: (a) akermanite (AKT) and MoS₂-modified AKT (MS-AKT) scaffolds with or without NIR treatment (808 nm, 0.6 W cm⁻², 10 min); (b) MS-AKT scaffolds with different irradiation duration (808 nm, 0.6 W cm⁻²); (c) MS-AKT scaffolds under different temperature (808 nm, 10 min); (d) MS-AKT scaffolds with different irradiation times (808 nm, 0.6 W cm⁻², 10 min). (*P<0.05, **P<0.01, ***P<0.001). SEM (e–h, cells shown by red arrows), confocal LSM (i–l; red: cytoskeleton; blue: cell nuclei) and live/dead staining (m-p; green: live cells; red: dead cells) images of Saos-2 cells in MS-AKT scaffolds with laser irradiation (e, i and m) and without laser irradiation (f, j and n), AKT scaffolds with laser irradiation (g, k and o) and without laser irradiation (h, l and p). MS-AKT scaffolds effectively killed tumor cells, showing excellent in vitro anticancer effect under NIR irradiation.