Table 1 Mechanical properties of enzyme-encapsulating hydrogels

From: A bioinspired dual-crosslinked tough silk protein hydrogel as a protective biocatalytic matrix for carbon sequestration

Supporting material

Enzyme

Testing method

Mechanical performance

Reference

Synthetic polymer

 Fmoc-TyrOH, N,N-dimethylacrylamide, and N-hydroxy-phthalimide

Glucose oxidase

Compression test

~206 kPa at 20% straina

17

 Polyethyleneimine

Penicillin G acrylase

Compression test

3.9−16.8 kPab

18

Natural peptides blended with synthetic polymers

 Nap-Phe-Phe-Lys-acrylic acid and poly(ethylene glycol) methacrylate

Glucose oxidase

Horseradish peroxidase

Compression test

7 kPa at 80% straina

20

Natural polymer

 Nap-Gly-Phe-Phe-pTyr, Nap-Gly-Phe-Phe-Tyr-Glu-ss-Glu-Gly, and alginate

Phosphatase

Lactase

Rheology test

12.3−22.2 kPab

19

 Alginate

Phosphatase

Lactase

Rheology test

12.1 kPab

19

 Silk fibroin

Carbonic anhydrase

Compression test

11 MPa at 40% straina

This work

  1. aMechanical performance was expressed as compressive modulus.
  2. bMechanical performance was expressed as compressive strength when fracture of the hydrogel occurred.
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