Abstract
Aim:
To investigate the ability of drug-loaded N,O-carboxymethyl chitosan (CMCS) hydrogels to modulate wound healing after glaucoma filtration surgery.
Methods:
The drug-loaded CMCS hydrogels were in situ synthesized using genipin as the crosslinker in the presence of 5-fluorouracil (5FU) or bevacizumab. Their structures were characterized by FTIR, ultraviolet-visible (UV-vis) spectroscopy and scanning electron microscopy (SEM). In-vitro drug release experiments and in vivo evaluation in rabbits were performed.
Results:
The results of FTIR, UV-vis spectroscopy and SEM analyses indicated that 5FU was encapsulated into the CMCS hydrogels that were crosslinked by genipin. The in vitro drug release experiments showed that nearly 100% of 5FU was released from the drug-loaded hydrogels within 8 h, but less than 20% bevacizumab was released after 53 h. The in vivo evaluation in rabbits indicated that the drug-loaded CMCS hydrogels were nontoxic to the cornea and were gradually biodegraded in the eyes. Furthermore, the drug-loaded CMCS hydrogels effectively inhibited conjunctival scarring after glaucoma filtration surgery and controlled postoperative intraocular pressure (IOP).
Conclusion:
The drug-loaded CMCS hydrogels provide a great opportunity to increase the therapeutic efficacy of glaucoma filtration surgery.
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Acknowledgements
We are grateful for support from the National Natural Science Foundation of China (No 20974130, 20574089), the Fundamental Research Funds for the Central Universities of China (No 09lgpy14), the Natural Science Foundation of Guangdong Province, China (No 6021311) and the Science and Technology Planning Project of Guangdong Province, China (No 2009B020313001, 2007B031504006).
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Yang, Lq., Lan, Yq., Guo, H. et al. Ophthalmic drug-loaded N,O-carboxymethyl chitosan hydrogels: synthesis, in vitro and in vivo evaluation. Acta Pharmacol Sin 31, 1625–1634 (2010). https://doi.org/10.1038/aps.2010.125
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DOI: https://doi.org/10.1038/aps.2010.125
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