Abstract
Aim:
To investigate the cytotoxic effects of four cyclic bisbibenzyls, Riccardin C (Ric), Pakyonol (Pak), Marchantin M (Mar), and Plagiochin E (Pla) against chemoresistant prostate cancer PC3 cells.
Methods:
Cell growth was assayed by MTT method, and apoptotic related protein Bcl-2 and Bax, poly(ADP-ribose) polymerase (PARP) were examined by Western blotting. Cell cycle and apoptosis of PC3 cells were evaluated with flow cytometry and morphologic examinations.
Results:
The four compounds inhibited proliferation and elicited cell death in a dose- and time-dependent manner with IC50 values of 3.22 μmol/L for Ric, 7.98 μmol/L for Pak, 5.45 μmol/L for Mar, and 5.99 μmol/L for Pla, respectively. Furthermore, exposed to these chemicals caused a decrease in the antiapoptotic protein Bcl-2 and an increase in proapoptotic Bax expression. PARP cleavage and caspase-3 activity were also observed.
Conclusion:
The results suggest that cyclic bisbibenzyls could be used for the development of novel therapeutic chemicals against prostate cancer.
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Abbreviations
- PI:
-
propidium iodide
- PARP:
-
poly(ADP-ribose) polymerase
- Ric:
-
Riccardin C
- Pak:
-
Pakyonol
- Mar:
-
Marchantin M
- Pla:
-
Plagiochin E
- AR:
-
androgen receptor
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (30772594), Shandong Provincial Key Research Foundation (2006GG1102023) and Shandong Scientific Technology Program (2008GG10002042).
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Xu, Ah., Hu, Zm., Qu, Jb. et al. Cyclic bisbibenzyls induce growth arrest and apoptosis of human prostate cancer PC3 cells. Acta Pharmacol Sin 31, 609–615 (2010). https://doi.org/10.1038/aps.2010.37
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DOI: https://doi.org/10.1038/aps.2010.37
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