Abstract
Aim:
To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions.
Methods:
Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release.
Results:
CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulation of peritoneal mast cells in guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P>0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P<0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL-2H3 cells. β-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with 1 mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P<0.01).
Conclusion:
CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.
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Acknowledgements
This work was supported by the Chinese Medicine Industry Research and Special Project (No 200707008) and by the Natural Science Foundation of Zhejiang Province (No Y206846).
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Huang, Fh., Zhang, Xy., Zhang, Ly. et al. Mast cell degranulation induced by chlorogenic acid. Acta Pharmacol Sin 31, 849–854 (2010). https://doi.org/10.1038/aps.2010.63
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DOI: https://doi.org/10.1038/aps.2010.63
