Abstract
Aim:
PRD1-BF-1-RIZ1 homologous domain containing protein-16 (PRDM16) is a cell-autonomous transcriptional component that stimulates the development of brown fat cells. The aim of this study was to investigate the contribution of genetic variants of PRDM16 to obesity-related phenotype variations in Chinese.
Methods:
A total of 3204 subjects (consisting of 400 male-offspring nuclear families, 401 female-offspring nuclear families, and 729 unrelated older males) were recruited. Ten tag single nucleotide polymorphisms (SNPs) within the PRDM16 gene were genotyped using multiplex quantitative real-time PCR by Taqman assay. Body compositions were measured by dual-energy X-ray absorptiometry (DXA). The associations of the SNPs with the obesity-related phenotypes were analyzed using the quantitative transmission disequilibrium test (QTDT), GLM-ANOVA and PLINK statistical methods.
Results:
Rs2236518 was the only SNP that was associated with BMI in young (aged 20–40 years) males (P=0.011) using QTDT, and in the older men (aged 50–80 years) (P=0.003) using GLM-ANOVA. No significant associations were detected in the females. Nor was a relationship found between any haplotype and obesity-related phenotypes. When PLINK was used, no significant relationship was detected between 10 SNPs and obesity-related phenotypes in any of the studied cohorts.
Conclusion:
Rs2236518 is associated with BMI in the young males (using QTDT), and the older males (using GLM-ANOVA).However, the result is not confirmed using PLINK. The discrepancy needs to be further addressed.
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Acknowledgements
The study was supported by grants from the National Natural Science Foundation of China (No 81170803, 81070692, 81000360, and 30800387), Shanghai Rising-Star Program (No 11QA1404900), Shanghai Natural Science Foundation (No 11ZR1427300), and Academic Leaders in Health Sciences in Shanghai (No XBR2011014).
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Yue, H., He, Jw., Ke, Yh. et al. Association of single nucleotide polymorphism Rs2236518 in PRDM16 gene with BMI in Chinese males. Acta Pharmacol Sin 34, 710–716 (2013). https://doi.org/10.1038/aps.2012.201
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DOI: https://doi.org/10.1038/aps.2012.201
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