Figure 3
Symmetric and asymmetric division of P6C cells. (A) Oct3/4 promoter activation in P6C and SW480 cells. P6C and SW480 cells were stably transfected with the pOct3/4 promoter-EGFP construct. Oct3/4 promoter activation and CD45 expression were measured by fluorescence intensity using a flow cytometer. (B) Self-renewal of an Oct3/4 positive P6C cell. A single Oct3/4 positive P6C cell was seeded in a 6-well plate and observed under a microscope. Pictures were taken every 24 h to detect holoclone formation. Scale bars, 50 μm. (C) Asymmetric division of an Oct3/4 positive P6C cell. A meroclone from a single Oct3/4 positive P6C cell underwent asymmetric division at the 2-cell stage. Pictures were taken every 24 h. Scale bars, 50 μm. (D) Distinct meroclone, paraclone and holoclones derived from Oct3/4 positive cells. Cell morphology in an Oct3/4 partial positive clone (top) showed the tight adhesion of the Oct3/4-positve cells and the loosen interaction of the Oct3/4-negative cells. Two side-by-side clones indicating that the paraclone is Oct3/4-negative and the holoclone is Oct3/4-positive (bottom). Scale bars, 200 μm.
