Figure 4
Karyotype and p53 mutations of P6C cells. (A) Representative karyotype of the P6C cells. Approximately 73% of the P6C cells possessed 59 chromosomes. Every chromosome was stained with a different color through FISH analysis. (B) The 72P to 72R mutation in a p53 allele of the P6C cells (passage 120). p53 cDNA was reverse transcribed from mRNA and cloned into a T-easy vector prior to sequencing (top). The C to G mutation was confirmed using Chromos Map (bottom). (C) A 117-bp insertion in the p53 cDNA from P6C cells, which resulted in a premature “TGA” stop codon after 25 amino acids. (D) p53 alleles from passage 4 and passage 120 P6C cells. The cDNA was reverse transcribed from mRNA, and the p53 gene was amplified by PCR before being inserted into the T-vector. The statistical analysis of the p53 alleles was performed by sequencing 10 to 22 constructs from each sample.
