Figure 2
DMU-212 inhibits cell viability and induces apoptosis in HUVECs. (A) Cells were treated with various doses of DMU-212 (0, 5, 10, 20, 40, or 80 μmol/L) in the presence of VEGF (10 ng/mL) for 24 or 48 h. Cell viability was determined by MTT assay. (B) Morphological micrographs were obtained under a phase-contrast microscope (×100). (C) Cell apoptosis was detected by TUNEL assay at 24 or 48 h. Bar=40 μm. (D) Histogram shows the ratio of TUNEL-positive cells. (E) Cells were treated with (20 μmol/L) or without DMU-212 in the presence of VEGF (10 ng/mL) for 24 or 48 h. After treatment, total cell lysates were prepared and analyzed for cleaved PARP, cleaved caspase 3, cleaved caspase 9, Bcl-2, and survivin by Western blot. In each case, the membrane was stripped and reprobed with anti-α-tubulin antibody to confirm equal protein loading. Data are expressed as the mean ±SEM of three independent experiments. bP<0.05, cP<0.01 vs control.
