Figure 3
DMU-212 inhibits VEGF-induced migration and capillary structure formation of HUVECs. (A) The effect of DMU-212 (0, 5, 10, 20, 40, or 80 μmol/L) on the migratory potential of VEGF-treated HUVECs was analyzed by wound healing assay. Top photos were taken immediately after scraping. Middle photos were taken 24 h after scraping. Bottom photos were taken 48 h after scraping. These photos were obtained under a phase-contrast microscope (×40). Histogram shows the cell migration distance data. (B) The effect of DMU-212 (0, 5, 10, 20, 40, or 80 μmol/L) on VEGF-treated tube formation of HUVECs was examined by plating cells on Matrigel. Histogram shows the total length of tubes after 24 h of treatment. These photos were obtained under a phase-contrast microscope (×100). Data are expressed as the mean±SEM of three independent experiments. bP<0.05, cP<0.01 vs control.
