Figure 4
DMU-212 inhibits VEGF-induced angiogenesis in vivo. (A) Representative photographs of Matrigel plugs containing 100 ng/mL VEGF with or without 20 μmol/L DMU-212. Plugs were removed from C57BL/6 mice at 2 weeks post-implantation. (B) Immunofluorescent labeling of microvessels with anti-CD31 antibody. Bar=40 μm. Histogram shows quantitative analysis of vessel density within Matrigel plugs. Microvessel density was defined as the ratio of vessel area CD31-positive/Matrigel plug area in 200× fields. (C) The effect of DMU-212 (0, 5, 10, 20, 40, or 80 μmol/L) on the newly formed blood vessels was examined by chick CAM assay. Histogram shows the relative number of newly formed blood vessels on disks. Assays for each test sample were carried out using 15 eggs. Data are expressed as the mean±SEM of three independent experiments. bP<0.05, cP<0.01 vs control.
