Figure 1
FFA4 expression in human colonic epithelial cells. (A) RT-PCR analyses of FFA1 and FFA4 expression in HCT116 and HT-29 human colon epithelial cells. GAPDH was used as the loading control. (B) RT-PCR analyses of long- and short-form FFA4 expression in HCT116 and HT-29 human colon epithelial cells. The long- and short-form FFA4 were used as positive controls. (C and D) Fluorescence confocal microscopy of HCT116 and HT-29 cells, respectively. FFA4 and β-arrestin 2 protein expression was detected with specific antibodies 30 min after exposing the cells to αLA, EPA, or DHA (50 μmol/L).
