Figure 4
Effects of 2-APB, 8-bro-cADPR, DAB or thapsigargin on the αLA-induced Ca2+ responses in human colon epithelial cells. In (A) and (C), HCT116 and HT-29 cells were pretreated with vehicle or 2-APB (100 μmol/L, 15 min), then treated with 50 or 100 μmol/L αLA. In (B) and (D), HCT116 and HT-29 cells were pretreated with vehicle, 8-bro-cADPR (100 μmol/L, 20 min), DAB (1 μmol/L, 30 min) or thapsigargin (1 μmol/L, 30 min), then treated with 50 or 100 μmol/L αLA. Percentage of the αLA-induced Ca2+ response compared with digitonin. The data are presented as the mean±SEM of three independent experiments. bP<0.05, cP<0.01 versus non-treated cells.
