Figure 2

IL-37b expression was confirmed in Ad-IL-37b-transduced MSCs. (A) IL-37b mRNA expression was evaluated by RT-PCR 48 and 72 h after Ad-IL-37b transfection of MSC. The IL-37b RT-PCR data were normalized to mouse GAPDH. (B) Western blotting analysis of IL-37b in MSC-IL-37b 48 and 72 h after Ad-IL-37b transfection. (C) IL-37b protein in the culture supernatant of MSC-IL37b was detected by ELISA. mean±SEM. n=3. bP<0.05.