Figure 2
LX2343 alleviated STZ-induced OS and ameliorated mitochondrial dysfunction. (A–D) Decreased ROS levels in LX2343-treated SH-SY5Y and primary neuronal cells. DCFH-DA fluorescence assayed by fluorescence and fluorimetry (one-way ANOVA, Dunnett's multiple comparison test. *P<0.05, **P<0.01 versus STZ. ##P<0.01 versus DMSO), scale bar: 100 μm. (E, F) Cells were stained with JC-1, and the mitochondrial membrane potential was detected (Δψ=525:590/490:530; one-way ANOVA, Dunnett's multiple comparison test. **P<0.01 versus STZ. ##P<0.01 versus DMSO). (G) Transmission electron microscopy images of mitochondria in SH-SY5Y cells, n=4, scale bar: 0.5 μm. (H, I) The intracellular ATP level was detected using a microplate reader (one-way ANOVA, Dunnett's multiple comparison test. **P<0.01 versus STZ. ##P<0.01 versus DMSO). All values were presented as the means±sem (n=3). Data were obtained from three independent experiments.
