Figure 4
LX2343 regulated the apoptosis-relevant proteins. (A–D) Western blot and quantification results demonstrated that LX2343 increased Bcl-2 or Bcl-xl protein level, increased Bad phosphorylation, decreased Bax protein level, and inhibited caspase 3 cleavage in SH-SY5Y and primary neuronal cells (one-way ANOVA, Dunnett's multiple comparison test, *P<0.05, **P<0.01 versus STZ, #P<0.05, ##P<0.01 versus DMSO). (E, F) Cytosolic and mitochondrial fraction were prepared, and cyt c distribution was analyzed (one-way ANOVA, Dunnett's multiple comparison test, *P<0.05 versus STZ, #P<0.05, ##P<0.01 versus DMSO). All values were presented as mean±SEM (n=3). GAPDH was used as loading control in Western blot assays. Data were obtained from three independent experiments.
