Figure 6
LX2343 had no effects on CDK5 pathway. (A–D) Western blot and quantification for the protein level of CDK5, p35 and p25 in SH-SY5Y and primary neuronal cells lysate (one-way ANOVA, Dunnett's multiple comparison test, compared versus STZ, #P<0.05, ##P<0.01 versus DMSO). (E) LX2343 had no effects on CDK5/p25 activity in vitro. PHA-793887: CDK5 inhibitor (one-way ANOVA, Dunnett's multiple comparison test, P=0.8967 versus DMSO; PHA-793887, t test, **P<0.01 versus DMSO). All values were presented as mean±SEM (n=3). GAPDH was used as loading control in Western blot assays. Data were obtained from three independent experiments.
