Figure 8
LX2343 protected against neuronal apoptosis in ICV-STZ rats by inhibiting JNK/p38 signaling pathway. (A) Representative micrographs of TUNEL-positive cells (green) in the hippocampus and cortex of ICV-STZ rats, scale bar: 100 μm. (B) Statistical analysis of A (one-way ANOVA, Dunnett's multiple comparison test. *P<0.05, **P<0.01 versus SV. ##P<0.01 versus V). (C–F) Western blot and quantification results demonstrated that LX2343 reduced JNK, c-Jun, and p38 phosphorylation in cortical and hippocampal homogenates of ICV-STZ rats (one-way ANOVA, Dunnett's multiple comparison test: *P<0.05, **P<0.01 versus SV. #P<0.05, ##P<0.01 versus V). Rats treated with vehicle, SV: Rats ICV injected with STZ and treated with vehicle, SL: Rats injected with STZ and treated with 7 mg·kg−1·d−1 LX2343, SH: Rats injected with STZ and treated with 21 mg·kg−1·d−1 LX2343. All values were presented as the mean±SEM (n=10 per group). GAPDH was used as the loading control in Western blot assays. Data were obtained from three independent experiments.
