Figure 9
LX2343 effectively inhibited neuronal apoptosis involving inhibition of pro-apoptotic pathways and promotion of synapse integrity. (A–D) Western blot and quantification results demonstrated that LX2343 increased Bcl-2 or Bcl-xl protein level, increased Bad phosphorylation, decreased Bax protein level, and inhibited caspase3 cleavage in cortex or hippocampus homogenates of ICV-STZ rats (one-way ANOVA, Dunnett's multiple comparison test: *P<0.05, **P<0.01 versus SV, #P<0.05, ##P<0.01 versus V). (E–H) Western blot and quantification results demonstrated that LX2343 increased synaptophysin, PSD95 or VAMP2 protein levels in cortex or hippocampus homogenates of ICV-STZ rats (one-way ANOVA, Dunnett's multiple comparison test: *P<0.05, **P<0.01 versus SV, #P<0.05, ##P<0.01 versus V). V: Rats treated with vehicle, SV: Rats ICV injected with STZ and treated with vehicle, SL: Rats injected with STZ and treated with 7 mg·kg−1·d−1 LX2343, SH: Rats injected with STZ and treated with 21 mg·kg−1·d−1 LX2343. All values were presented as mean±SEM (n=10 per group). GAPDH was used as loading control in Western blot assays. Data were obtained from three independent experiments.
