Figure 2
MK-8776 enhances irradiation-induced DNA damage. (A–C) Immunofluorescence assay results and the numbers of γH2A.X foci in the three TNBC cell types; (D) Compared with the IR-alone group, the IR+MK-8776 group also exhibited significantly increased percentages of more than 10 foci in the MDA-MB-231 (39.67%±3.51% vs 33.0%±2%, P=0.046), BT-549 (51.67%±3.06% vs 35.33%±1.33%, P=0.001) and CAL-51 (39.33%±2.52% vs 32%±%3.61, P=0.045) cell lines. (E) Markedly increased phosphorylation of Chk1 at ser296 was observed after irradiation, which was inhibited by MK-8776. No detectable phosphorylation of Chk1 at ser345 was observed after irradiation. A dramatic increase in γH2A.X was observed after irradiation with or without MK-8776. Error bars represent SDs. **P<0.05.
