Figure 6 | Acta Pharmacologica Sinica

Figure 6

From: Two birds, one stone: dual targeting of the cancer cell surface and subcellular mitochondria by the galectin-3-binding peptide G3-C12

Figure 6Figure 6

(A) Western blot analyzed the galectin-3 expression level after treated inhibitor MCP in PC-3 cells and mitochondria. Samples were run at equal protein concentration and immunostained against cell markers (GAPDH) and mitochondrial marker (COX IV). (B) Western blot analyzed the galectin-3 protein translocated in mitochondria under the stimulation of KLA or KLA puls MCP. (C) Z-stack images of subcellular distribution of galectin-3 after treatment with 100 μmol/L KLA plus MCP. (D) Representative images of mitochondrial distribution of FITC-labeled G3-C12-HPMA-KLA pretreated with inhibitor MCP. (E) Flow cytometry analyzed the accumulation of G3-C12-HPMA-KLA plus MCP in mitochondrial of PC-3 cells. Data are presented as mean±SD (n=3). *P<0.05.

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