Figure 4 | Acta Pharmacologica Sinica

Figure 4

From: Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3′UTR in human liver cells

Figure 4

PTEN-sh-3p21 targets the 3′UTR of protein phosphatase PPP2CA or PTEN. (A) A model of the PPP2CA locus, showing putative target sites for PTEN-sh-3p21. (B) Luciferase reporter constructs showing the wild type and mutant PPP2CA mRNA 3′UTRs. (C) The effect of PTEN-sh-3p21 on the PPP2CA mRNA 3′UTR was examined by luciferase reporter assay in 293T cells. (D) The expression of PPP2CA and PTEN was tested by Western blot analysis in L-O2 cells treated with PTEN-sh-3p21. Mean±SD. n=3. Each experiment was repeated three times. NSP>0.05, **P<0.01 vs mimics NC, unpaired Student's two-tailed t-test.

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