Figure 9
THA promoted Aβ clearance involving both PI3K/AKT/mTOR and AMPK/Raptor/mTOR signaling-mediated autophagy activation. A Western blot assay was carried out for the cortices of the transgenic mice. (A) The levels of P-PERK, PERK, P-eIF2α, eIF2α, sAPPβ, and BACE1; (B) Densitometry analysis of (A). (C) BACE1 mRNA levels were detected by RT-PCR assays. (D) The levels of p62, LC3II, P-ULK1 and ULK1; (E) Densitometry analysis of (D); (F) The levels of P-mTOR, mTOR, P-P70S6K, and P70S6K; (G) Densitometry analysis of (F). (H) The levels of P-PI3K, PI3K, P-AKT, and AKT; (I) Densitometry analysis of (H). (J) The levels of P-AMPK, AMPK, P-Raptor and Raptor; (K) Densitometry analysis of (J); GAPDH was used as a loading control in the Western blot assays. NT-V, non-transgenic vehicle group; T-V, transgenic vehicle group; T-THA, transgenic mice administration with 300 mg THA/kg per day group. Values are the mean±SEM. t test. n=4. #P<0.05, ##P<0.01 compared with T-V.
