Figure 4A
Res-006-induced cell death is prevented by mitochondria-targeted superoxide scavenger. (A) Flow cytometry analysis of the accumulated superoxide levels using DHE (15 μmol/L) dye in Res-006 (65 μmol/L)-treated cells. A representative image from three independent experiments is shown in the left panel. The graph shows the relative fluorescence levels of three independent samples per group. The data are expressed as the mean±SEM (n=3 mice per treatment). (B-D) HepG2 cells were treated for 24 h with Res-006 (65 μmol/L) only or Res-006 (65 μmol/L) and TEMPOL (100–900 μmol/L) in (B), Res-006 (65 μmol/L) only or Res-006 (65 μmol/L) and Mito-TEMPO (100–1000 μmol/L) in (C), or Res-006 (65 μmol/L) only, Res-006 (65 μmol/L) and TEMPOL (500 μmol/L), or Res-006 and Mito-TEMPO (300 μmol/L) in (D). Cell viability was analyzed using the CCK-8 assay. The data are expressed as the mean±SEM of three independent experiments, *P<0.05 and ***P<0.001; untreated group vs each treated group, ##P<0.01 and ###P<0.001; Res-006-treated group vs each treated group, &&P<0.01; Res-006- and TEMPOL-treated group vs Res-006- and Mito-TEMPO-treated group. (E) HepG2 cells were treated with the indicated chemicals for 24 h, and cell viability was examined using confocal microscopy. The scale bar denotes 200 μm.
