Figure 5
Mitochondria-targeted superoxide scavenger prevents Res-006-mediated disruption of MMP. (A) Flow cytometry analysis of the accumulated superoxide levels in the presence of DHE (15 μmol/L) after a 2 h treatment with Res-006 (65 μmol/L) only, Res-006 (65 μmol/L) and TEMPOL (500 μmol/L), or Res-006 (65 μmol/L) and Mito-TEMPO (300 μmol/L). A representative image from three independent experiments is shown in the upper panel. The graph shows the relative fluorescence levels of three independent samples per group. The data are expressed as the mean±SEM, *P<0.05 and ***P<0.001; mock-treated group vs each treated group, #P<0.05 and ###P<0.001; Res-006-treated group vs each treated group, &P<0.05; Res-006- and TEMPOL-treated group vs Res-006- and Mito-TEMPO-treated group. (B) HepG2 cells were stained with Hoechst 33258 and JC-1 (2.5 μmol/L) after 2-h treatments with the indicated chemicals and analyzed using confocal fluorescence microscopy. The scale bar denotes 10 μm. (C) HepG2 cells were stained with Hoechst 33258 and MitoTracker Red after treatment with Res-006 (65 μmol/L) only, Res-006 (65 μmol/L) and TEMPOL (500 μmol/L), or Res-006 (65 μmol/L) and Mito-TEMPO (500 μmol/L). Samples were analyzed by fluorescence microscopy. The scale bar denotes 10 μm. Inset shows a magnified view of the area outlined in the white lined box.
