Figure 1
From: Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b
PT reduced cell proliferation and triggered caspase cascade apoptosis in EC cells. (A) The cytotoxicity of PT (from 25 to 100 mol/L) was detected by a CCK-8 assay; the proliferation ratio decreased in a dose-dependent manner (* means P<0.001 vs untreated control group 100%). (B) Caspase-3, -8, and -9 activities were detected by colorimetric assay. The relative activity of the caspases was measured as the ratio of optical density of the experimental group divided by the optical density of the control group, which was considered 1. Caspase-3, -8, and -9 were all activated by PT in a dose-dependent manner (* means P<0.001 vs untreated control group). (C) Annexin V/PI analysis showed that PT induces apoptosis in HTB-111 and Ishikawa cells. A higher concentration of PT was associated with an increased apoptosis ratio in EC cells.
