Figure 2
From: Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b
PT down-regulated miR-663b and indirectly up-regulated its target, BCL2L14. (A) Identification of the change in miR expression by microarray. The miR clusters are represented by red or green according to its score. The hierarchical clustering profile shows the variation of miRs in Ishikawa cells after treatment with IC50 of PT. (B) We detected the 9 downregulated miRs by qRT-PCR. miR-663b was decreased the most significantly. (C) When exposed to different concentrations of PT, miR-663b levels in HTB-111 and Ishikawa cells, measured by qRT-PCR, increased gradually. (D) TargetScan predicted that miR-663b targets BCL2L14 by binding to its 3′UTR. (E) The luciferase activities of wild-type of pMIR-BCL2L14, but not mutant pMIR-BCL2L14 or a negative control of empty plasmid, were reduced by miR-663b mimics (* means P<0.05). (F-G) The BCL2L14 mRNA (F) and protein (G) levels in both HTB-111 and Ishikawa cells changed depending on miR-663b levels. We also treated the EC cells with IC50 of PT and found that BCL2L14 increased in both mRNA and protein.
