Figure 2
Analytical performance. (a) Multiplex standard curves using Armored RNA Quant technology. The four calibrators, each containing various levels of BCR–ABL1 (e1a2), ABL1 and Norm targets and covering 5 logs of linear dynamic range overall, were tested in triplicate. The three resulting four-point standard curves and corresponding R2 automatically generated by the 7500 instrument's software are shown. (b, c) Representative examples of analytical sensitivity and linearity with five synthetic RNAs prepared by in vitro transcription or three leukemic cell line RNAs diluted in a background of HL-60 total RNA (1500 ng input). The graphs show the mean values obtained from duplicate testing (108, 106 or 104 copies of synthetic target; cell lines undiluted or diluted 1 in 102 or 1 in 104), triplicate testing (100 or 50 copies of synthetic target; cell lines diluted 1 in 105) or quadruplicate testing (10 copies of synthetic target; cell lines diluted 1 in 106). (d) Results from duplicate testing using 40 independent cell line RNA dilutions at 1500 ng input. The graph shows the percent ratio for the second measure plotted against the percent ratio of the first measure and the theoretical equality line (first measure=second measure).
