Figure 4

Evaluation of reference materials. (a) Ct values obtained for each of the four samples tested in triplicate with the BCR/ABL1 Quant assay (1000 ng input). The linear regression curve and corresponding equation and R2 for BCR–ABL1 are shown. (b) Correlation between the mean BCR–ABL1 and ABL1 percent ratios obtained for each sample with the multiplex assay. The linear regression curve and corresponding equation and R2 are shown. The mean IS percent ratios reported by White et al.11 using three different endogenous control genes (ABL1, BCR or GUS) are also shown.