Figure 4 | Blood Cancer Journal

Figure 4

From: Resistance of MLL–AFF1-positive acute lymphoblastic leukemia to tumor necrosis factor-alpha is mediated by S100A6 upregulation

Figure 4

Western blotting analysis of lysate from MLL–AFF1-positive leukemia cell lines (SEM and RS4;11) under the MLL–AFF1 siRNA/S100A6 siRNA/control siRNA in the presence of TNF-α (5 ng/ml). All of acetyl-p53/p53 ratio, cleaved caspase 8 and cleaved caspase 3 expression were increased in cells treated with MLL–AFF1 siRNA or s100A6 siRNA in comparison with those treated with control siRNA in the presence of TNF-α (5 ng/ml). S100A6 expression was inhibited by both MLL–AFF1 siRNA and S100A6 siRNA in comparison with control siRNA. MLL–AFF1 expression was inhibited by MLL–AFF1 siRNA in comparison with control siRNA, but MLL–AFF1 expression was not inhibited by S100A6 siRNA. Densitometry measurements of control siRNA were standardized as 1.00.

Back to article page