Figure 6
NPDox/VLA4−pep inhibits adhesion of MM cells to fibronectin and overcomes CAM-DR. (a) Calcein-labeled NCI-H929 MM cells were allowed to adhere to fibronectin-coated plates alone, or with increasing concentrations of NPVLA4−pep. Nonadherent cells were removed by washing with PBS, and adherent cells were quantitated in a fluorescence multi-well plate reader. Data represents means (±s.d.) of triplicate experiments. *P<0.05, **P<0.01 when compared with control. (b) NCI-H929 cells were allowed to adhere to fibronectin- or BSA-coated plates for 1 h, and then treated with equivalent Dox concentrations of NPDox/VLA4−pep, or free Dox for 72 h. Cell viability was assessed by cell counting kit-8 (CCK-8), and data represent means (± s.d.) of triplicate cultures. (c) An alternate illustration of data presented in (b).
