Figure 2

p89c-Mybex9b binds the cyclin B1 and SLUG promoter. Chromatin immunoprecipitation assays show binding (detected by real-time PCR) of p75c-Myb and p89c-Mybex9 to a segment of the cyclin B1 promoter (a, left) and SLUG promoter (b, left). Error bars denote s.d. of the means of two experiments performed in triplicate. (a and b, right): partial sequence of the human promoter of cyclin B1 (GenBank: U22364.1) and SLUG (GenBank: AB300659.1). Putative c-Myb-binding sites located in the 5′-untranslated region (italics) are underlined; primers used for real-time PCR are in bold.