Figure 5

Epigenetic environment and PolII recruitment at the c-myb promoter and first intron. ChIP-on-chip experiments were performed using chromatin obtained from HPC7 and FMH9 cells and antibodies directed against H3K4ac and H3K4me3 (a). Enrichment values were calculated as fold-enrichment over the mean intensity across the locus and expressed as log base 2. The position of the hypersensitive regions and c-myb exons 1 and 2 are indicated. Representation of X- ChIP experiments using an antibody against PolII and chromatin derived from HPC7 and FMH9 (b). The relative enrichments associated with the HS-B and HS-D regions were determined by q-PCR against the control IgG ChIP material. Ratio between the transcriptional levels of exon 2 compared with exon 1 of the c-myb gene performed in HPC7, FMH9, HPC7 D4 and HPC7 D6 (c). The table represents the relative level of expression measured at exon 1 (ex1) and exon 2 (ex2) of the c-myb gene. q-PCR results were normalized against the 18S gene.