Figure 5

Inhibition of MerTK impairs the clonogenic potential of the Jurkat cell line. (a) Jurkat parental and shRNA derivative cells were plated in methylcellulose media, grown for 8 days, and colonies were counted. (b) Analysis of methylcellulose colony formation in Jurkat parental and shRNA derivative cells transduced with an empty (pLNCX) or a MerTK expression vector. Error bars represent the s.e. of the mean of at least three independent experiments. Statistical comparison vs shCntrl1 by one-way repeated measures analysis of variance followed by Bonferroni posttests. The differences in colony number between the Jurkat parental, shCntrl1 and shMer1A+MerWT lines were not statistically significant.