Figure 5
From: Anti-tumor activity of obinutuzumab and rituximab in a follicular lymphoma 3D model
GA101 affects MALC signaling pathways. Cell signaling was analyzed as described in Materials and Methods. Dot plots represent all encoded conditions discriminated according to the CBD450 fluorescence of either 2D RL cells treated for 72 h with 10 μg/ml antibody (a) or MALC after 20 days of antibody treatment (b). Phosphospecific stainings were analyzed for each gated condition and heatmaps represent relative levels of phospho-Akt/Akt, phospho-Syk/Syk and phospho-PLCγ2/PLCγ2 for one representative experiment in RL treated or not for 72 h or in MALC treated or not at day 20. Analysis and representation were performed with Cytobank. Histograms represent the relative percentage of phosphorylated proteins in treated 2D (a) or 3D (b) cells compared with UT cells. Results represent mean±s.d. of three independent experiments *P<0.05. Western blot analysis of p70S6K phosphorylation was performed in 2D RL cells treated or not with 10 μg/ml antibody for 72 h (a).
