Figure 4

Increase in serum Ig levels and emergence of serum paraproteins in the course of PCT development. (a, b) Serum Ig levels were determined using isotype-specific enzyme-linked immunosorbent assay (ELISA). Normal C mice (black) were compared with IL6Myc-Tg mice at early (white columns), mid (hatched) and late (grey) stages of tumor progression as defined in Figure 5a. Statistical comparisons of stages of tumor progression were carried out using the two-tailed t-test; P-values are indicated where the threshold of significance (5%) was reached. (c) Paraproteins (indicated by red arrowheads) were detected using serum protein electrophoresis. Age of mice and ratio of γ-globulins to albumin (G/A) are indicated at the bottom.