Figure 5

BTK knockdown in MM cells reduces their adhesion, impairs their migration and alters their expression of genes associated with adhesion, migration and growth. (a) Bioluminescence analysis of BTK-KD and CONT cells adhering to fibronectin-coated plates alone (left) or with MSCs (right), expressed as percent adherent cells from total cells. (b) Migration of BTK-KD and CONT cells toward SDF-1 (30 nM), expressed as percent changes compared with spontaneous migration without SDF-1. (c, d) Selected overexpressed (high) or underexpressed (low) genes in BTK-KD tumor cells recovered from SCID-rab mice (see Supplmentary Table S1 for the complete list of genes). Bolded gene symbols indicate their expression pattern was originally present in BTK-KD cells cultured in their standard in vitro condition (see Supplmentary Table S2 for the complete list of genes).