Figure 1

CG-rich region at the 5′ end of the PRDM1 gene. (a) The 5′ region of the PRDM1 gene is predicted to contain a CG island spanning the promoter region and exon 1. The genomic region analyzed by bisulfite sequencing (−325 to +224) is indicated. The filled region indicates the open reading frame; the bent arrow denotes the transcription start site. (b) The sequence at the 5′ end of PRDM1, including the region −325 to +224 analyzed by bisulfite sequencing, is depicted. The three portions of sequence that were separately analyzed in formalin-fixed, paraffin-embedded primary BLs are highlighted in gray. The 41 CpG dinucleotides interrogated are numbered within the sequence. The boundaries of the predicted CpG island are indicated by horizontal arrows.