Figure 6

PRDM1 induces cell cycle arrest but not apoptosis in BL cells. (a) BL cells (Daudi and P3HR1) were transfected with PRMD1-PIG or PMSCV-PIG plasmids. Western blotting for PRDM1 was performed 48 h posttransfection. Lamin B was included as a loading control. (b) Apoptosis of GFP-positive cells measured by Annexin V/7ADD at 48 h posttransfection. Error bars indicate the s.e.m. from three independent experiments. NS, not significant. (c) Cell cycle analysis of GFP-positive populations at 48 h posttransfection. Transfected BL cells were labeled with 10 μM BrdU for 2 h, followed by intracellular staining with BrdU and 7-amino-actinomycin D (7-AAD) and flow cytometric analysis. Percentages of cell cycle events at different phases were calculated using the FlowJo software and the graph was plotted using Prism 6 software (GraphPad, La Jolla, CA, USA). Error bars denote the s.e.m. from three independent experiments, and the P-values were indicated. A representative graph was shown.