Figure 1
From: Hypoxia promotes stem cell-like phenotype in multiple myeloma cells
Hypoxia promotes dedifferentiation, decreases cell proliferation, induces G1-cell cycle arrest, but not apoptosis of MM cells. The effect of hypoxia (1% O2) on (a) CD138-PerCP-Cy5.5, CD20-PE, CD45-APC, CD19-FITC and CD34-PE expression, analyzed by flow cytometry and shown as fold change of normoxia in MM1s, H929, RPMI8226, OPM1, U266 cell lines; (b) proliferation of MM cells analyzed by MTT (3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide) assay; (c) the expression of PI3K signaling pathway and stress kinases by immunoblotting; (d) cell cycle analysis tested by propidium iodide staining and flow cytometry and shown as the percentage of cells in G1, S and G2/M phases; (e) the expression of cell cycle-related proteins detected by immunoblotting; (f) cell apoptosis performed using APO 2.7 (PerCP-Cy5.5) staining and shown as the percentage of apoptotic (APO 2.7-positive) cells; and (g) apoptosis-related proteins detected by immunoblotting.
