Figure 2
From: Hypoxia promotes stem cell-like phenotype in multiple myeloma cells
Hypoxia induces rapid tumor initiation and drug resistance to bortezomib and carfilzomib in MM cells. MM1s-Luc-GFP cells were incubated in normoxia or hypoxia for 24 h and injected intravenously into three severe combined immunodeficiency (SCID) mice per condition at the concentration of 2 × 106 cells per mouse. The effect of hypoxia (1% O2) on tumor initiation and tumor progression in SCID mice was monitored for 5 weeks using bioluminescent imagining (BLI) and shown as the average of the luminescent signal of three mice (a), and as representative BLI pictures of mice injected with normoxic or hypoxic cells at weeks 2, 3, 4 and 5 (b). The effect of hypoxia on drug resistance in MM cells shown as the proliferation of MM cells after the treatment with 5 nm bortezomib or 5 nm carfilzomib, analyzed using MTT (3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide) assay (c).
