Figure 1
From: RUNX1 haploinsufficiency results in granulocyte colony-stimulating factor hypersensitivity
Runx1+/− BM cells show G-CSF hypersensitivity in vitro. (a) Colony-forming potential of 50 000 whole-BM cells in the presence of indicated cytokine: 10 ng/ml IL-3, 500 ng/ml IL-6, 10 ng/ml murine G-CSF, 10 ng/ml granulocyte-macrophage CSF (GM-CSF) and 100 ng/ml stem cell factor (SCF). (n= 3 mice/genotype). (b) Colony-forming potential of 10 000 whole-BM cells in the presence of increasing concentration of G-CSF. (n= 3 mice/genotype). (c) Colony-forming of purified progenitor compartments in the presence of 100 ng/ml G-CSF. All data represent mean±s.d. (n= 3 mice/genotype). Three independent experiments were performed. CMP, common myeloid progenitors (CD34+FcγRII-KL); GMP, granulocyte-macrophage progenitors (CD34+FcγRII+KL); MEP, megakaryocyte erythrocyte progenitors (CD34-FcγRII-KL). Asterisks represent significant differences (*P<0.05, **P<0.01 and two-tailed Student’s t-test).
