Figure 2
From: RUNX1 haploinsufficiency results in granulocyte colony-stimulating factor hypersensitivity
Runx1+/− mice exhibit acute G-CSF hypersensitivity in vivo. (a–c) Graphical representation of flow cytometric analysis of the HSPCs and myeloid progenitor compartments (a), individual HSPC compartments (b) and myeloid progenitors (c) in the BM 24 h after in vivo G-CSF stimulation (250 μg/kg/day for 3 days). Percentages shown are frequencies of viable lineage− population. Data from at least two independent experiments are shown. (Runx1+/+mice phosphate-buffered saline (PBS) control, n=4; Runx1+/− mice PBS control, n=4; Runx1+/+mice G-CSF-treated, n= 6; Runx1+/+mice G-CSF-treated, n=6). (d) G-CSF mobilization assay of cells from Runx1+/− mice and Runx1+/+ mice. Time course of colony numbers in 20 μl PB after in vivo G-CSF stimulation (250 μg/kg/day for 3 days) is shown. (n=2/genotype). Two independent experiments were performed. (e) Representative plots and graphical representation (left and right panel respectively) of flow cytometric analysis of the HSPC compartments in the spleen 24 h after in vivo G-CSF stimulation (250 μg/kg/day for 3 days). Percentages shown are frequencies of viable lineage− population. (Runx1+/+ mice PBS control, n=4; Runx1+/− mice PBS control, n= 4; Runx1+/+mice G-CSF-treated, n= 6; Runx1+/+ mice G-CSF-treated, n=6). (f) Representative graphical representation of flow cytometric analysis of GMP frequency in the spleen 24 h after in vivo G-CSF stimulation (250 μg/kg/day for 3 days). All data represent mean±s.d. (Runx1+/+mice PBS control, n=3; Runx1+/− mice PBS control, n=3; Runx1+/+mice G-CSF-treated, n=3; Runx1+/+mice G-CSF-treated, n= 3). (g) Representative plots and graphical representation (left and right panel respectively) of flow cytometric analysis of the myeloid cells in the BM 24 shown (Runx1+/+mice PBS control, n=2; Runx1+/− mice PBS control, n=2; Runx1+/+ mice G-CSF-treated, n= 4; Runx1+/+mice G-CSF-treated, n=4). CMP, common myeloid progenitors (CD34+FcγRII-KL); GMP, granulocyte-macrophage progenitors (CD34+FcγRII+KL); MEP, megakaryocyte erythrocyte progenitors (CD34-FcγRII-KL). Asterisks represent significant differences (*P<0.05, **P<0.01 and two-tailed Student’s t-test).
